Genetic Characteristics of <i>Citrus Tristeza Virus</i> Isolates from Cultivated Citrus in China Based on Coat Protein Gene

Citrus tristeza virus (CTV) is an important citrus pathogen causing considerable economic loss to production. Knowledge on genetic evolutionary of the CTV population in China remains limited. In this study, 1439 samples were collected from nine citrus-producing areas China. The coat protein (CP) genes amplified by RT-PCR, and sequenced analyze evolution. Analysis base composition showed AU preference pattern, with GC content was lower than content. Nine populations clustered into one clade neighbor-joining (NJ) tree, indicative a close phylogenetic relationship among molecular variation (AMOVA) revealed that 77.72% variations observed populations, F ST value 0.223. values dN/dS neutrality test CP gene ranged 0.016 0.082 ?1.377 1.456, respectively, results suggesting all relatively constantly maintained under purifying selection. Our study demonstrated characteristics evolution China, provided theoretical basis for scientific control CTV.